Mimei Harnesses Biosynthetic Technology to Synthesize Pullulan

Mimei Biotechnology
Mimei Harnesses Biosynthetic Technology to Synthesize Pullulan
Shandong Mimei Biotechnology Co., Ltd., a leading pullulan manufacturer, has significantly improved pullulan production by addressing key challenges such as low yields, melanin removal, and high extraction costs. Utilizing innovative fermentation techniques and sustainable practices, Mimei boosts efficiency and reduces costs. Their eco-friendly methods ensure high-quality, versatile pullulan for diverse applications in cosmetics, medicine, food, and more.

Mimei Harnesses Biosynthetic Technology to Synthesize Pullulan


In the rapidly advancing field of biotechnology, Shandong Mimei Biotechnology Co., Ltd. stands at the forefront of innovation and sustainability. Established in 2019, our company has quickly become a leading manufacturer and supplier of pullulan and related bio-polysaccharide products. With a strong technical foundation and extensive manufacturing experience, we are dedicated to improving the production processes and reducing costs associated with pullulan synthesis. This article explores the innovative approaches we have adopted to address the challenges in pullulan production, highlighting our commitment to efficiency, cost-effectiveness, and environmental sustainability.

Company Profile

Shandong Mimei Biotechnology Co., Ltd. boasts a factory scale of over 100,000 square meters and an annual output capacity of 3,000 tons of pullulan. Our production capabilities extend to 10 billion pullulan hollow capsules and 80 million pullulan biopolysaccharide films annually. We have achieved ISO22000, ISO14001, and ISO45001 quality system certifications, alongside organic, kosher, and halal certifications, ensuring that our products meet the highest standards of quality and safety. Our mission is to drive the industrialization of biological synthesis of pullulan through the development of innovative technologies and sustainable practices.

Challenges in Pullulan Production

1. Low Product Yield

One of the primary challenges in the industrial production of pullulan is the relatively low product yield. The current process typically produces a sugar concentration of about 60 g/L. Increasing the yield is crucial as it directly impacts the fermentation and separation costs. At Shandong Mimei, we have made significant strides in enhancing yield through optimized fermentation conditions and the use of advanced biocatalysts. Our research and development efforts at the 5L fermentation tank level have resulted in an output of 109 g/L, with a yield reaching between 45 and 50%, significantly higher than the existing technology.

2. Difficulty in Removing Melanin

During the fermentation process, Aureobasidium pullulans, the primary microorganism used in pullulan production, secretes black or dark green substances similar to melanin. This melanin adheres firmly to pullulan, making it challenging to remove using ordinary methods. Our team at Mimei has developed innovative purification techniques that effectively separate melanin from pullulan, ensuring the purity of our final product. These methods include the use of advanced filtration systems and enzymatic treatments that target melanin without compromising the quality of pullulan.

3. High Cost of Polysaccharide Extraction and Drying

The extraction of polysaccharides from pullulan is a cost-intensive process, primarily due to the large amounts of ethanol required. Although ethanol can be recovered, the associated costs remain high. At Mimei, we are pioneering more cost-effective extraction methods that reduce the reliance on ethanol. By integrating alternative solvents and improving recovery processes, we have managed to lower the overall extraction costs significantly. Additionally, our drying techniques have been optimized to enhance efficiency and reduce energy consumption, further contributing to cost savings.

4. Low Conversion Rate

The current fermentation process for pullulan production has a conversion rate of about 40% to 50%, which is far below the theoretical conversion rate of 90%. Improving this conversion rate is essential for maximizing efficiency and reducing costs. Our research focuses on optimizing the fermentation conditions, including pH, temperature, and nutrient supply, to enhance the metabolic efficiency of Aureobasidium pullulans. Through these efforts, we have been able to achieve higher conversion rates, approaching the theoretical maximum, thus making the production process more efficient and cost-effective.

Innovative Solutions and Sustainable Practices

Development of Cheap Raw Material Technology

At Shandong Mimei, we are committed to reducing the costs of raw materials used in pullulan production. By sourcing renewable biomass hydrolyzate as our primary raw material, we not only lower the production costs but also contribute to environmental sustainability. This approach utilizes waste products from other industries, turning them into valuable inputs for our fermentation process.

Control of Fermentation Conditions and Processes

Precision control over fermentation conditions is crucial for optimizing pullulan yield and quality. We employ advanced monitoring and control systems that allow us to fine-tune the fermentation environment in real-time. This ensures that the microorganisms are provided with optimal conditions for growth and production, leading to higher yields and better product consistency.

Product Separation Optimization

Efficient separation of pullulan from the fermentation broth is another area where we have made significant improvements. Our separation techniques involve a combination of filtration, centrifugation, and selective precipitation, which ensure high purity and yield of the final product. These optimized processes not only improve the quality of pullulan but also reduce the overall production costs.

Green Catalytic Fermentation

Our commitment to sustainability is reflected in our adoption of green catalytic fermentation processes. By utilizing environmentally friendly catalysts and minimizing the use of harmful chemicals, we ensure that our production processes are safe for both the environment and our workers. This approach aligns with our broader goal of promoting sustainable and eco-friendly manufacturing practices.

Applications of Pullulan

Pullulan is a versatile biopolymer with a wide range of applications across various industries. Its excellent properties, such as biodegradability, non-toxicity, and film-forming ability, make it suitable for use in cosmetics, medicine, food, chemical industry, environmental protection, and pesticides.


In the cosmetics industry, pullulan is used as a film-forming agent and stabilizer. Its ability to create a smooth and flexible film makes it ideal for use in skin care and hair care products. Additionally, its biodegradability ensures that it does not contribute to environmental pollution.


Pullulan's non-toxic and biocompatible nature makes it suitable for use in medical applications. It is used in the production of capsules, drug delivery systems, and wound dressings. Its ability to be easily degraded and utilized by microorganisms in nature ensures that it does not cause any adverse effects on the environment.

Food Industry

In the food industry, pullulan is used as a food additive and packaging material. Its film-forming ability and biodegradability make it an excellent choice for creating edible films and coatings. Additionally, its non-toxic nature ensures that it is safe for consumption.

Chemical Industry

Pullulan is used in the chemical industry as a stabilizer and thickening agent. Its ability to form strong and flexible films makes it suitable for use in various industrial applications, including adhesives and coatings.

Environmental Protection

Pullulan's biodegradability and non-toxicity make it an excellent choice for use in environmental protection applications. It can be used in the production of biodegradable plastics and other environmentally friendly materials.


In the agricultural sector, pullulan is used as a carrier for pesticides. Its ability to form stable films ensures that the pesticides are effectively delivered and remain active for longer periods, improving their efficacy and reducing the need for frequent applications.


Shandong Mimei Biotechnology Co., Ltd. is committed to advancing the industrial production of pullulan through innovative technologies and sustainable practices. By addressing the challenges in pullulan production and optimizing our processes, we have significantly improved the efficiency and cost-effectiveness of our manufacturing operations. Our focus on sustainability ensures that our production methods are environmentally friendly, contributing to a greener future. With our strong technical capabilities and extensive manufacturing experience, we are well-positioned to continue leading the industry in the production of high-quality pullulan and related bio-polysaccharide products.

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